Insulin sensitivity studies can get complicated quickly, but we're here to help. See our recommendations below whether your study is for GTT, Hyperglycemic Clamp or Hyperinsulinemic-Euglycemic Clamp.
Insulin sensitivity studies can get complicated quickly, but we're here to help. See our recommendations below whether your study is for GTT, Hyperglycemic Clamp or Hyperinsulinemic-Euglycemic Clamp.
In this study design, a loading dose of glucose (gtt) or insulin (itt) is followed by intermittent blood sampling. The dose is typically administered orally, and since multiple blood samples are needed, an access port is the best tool to make catheter access quick and easy.
In this study design, glucose is infused intravenously with adjustments to reach a steady state. From there, intermittent blood samples are collected and analyzed to establish glucose levels. Since the mouse must be tethered, and low-stress, the CannuLock™ Catheter Access Button™ is an ideal tool.
This protocol relies on intravenous (IV) infusion of insulin to set a baseline, as well as variable rate infusion of glucose (into the same IV line). It's also common to incorporate an additional channel to dose an experimental compound. Intermittent blood sampling for glucose levels will determine the impact of the drug administration on the mouse.